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Monitoring of Immunoglobulin N- and O-glycosylation in Health and Disease.

Monitoring of Immunoglobulin N- and O-glycosylation in Health and Disease.

Protein N- and O- glycosylation are well-known co- and post-translational modifications of immunoglobulins. Antibody glycosylation on the Fab and Fc portion is understood to affect antigen binding and effector features, respectively. To check associations between antibody glycosylation profiles and (patho)physiological states in addition to antibody performance, superior applied sciences and strategies are required. In-depth structural characterization of antibody glycosylation often depends on the separation and tandem MS evaluation of launched glycans. Protein- and site-specific data, alternatively, could also be obtained by the MS evaluation of glycopeptides.

With the event of high-resolution mass spectrometers, antibody glycosylation evaluation on the intact or middle-up stage has gained extra curiosity, offering an built-in view of various post-translational modifications (together with glycosylation). Alongside the in-depth strategies, there’s additionally nice curiosity in sturdy, high-throughput methods for routine glycosylation profiling in biopharma and scientific laboratories. With an emphasis on IgG Fc glycosylation, a number of extremely sturdy separation-based methods are employed for this goal. On this overview, we describe current advances in mass spectrometric strategies, separation methods and orthogonal approaches for the characterization of immunoglobulin glycosylation in numerous settings. We put emphasis on the present standing and anticipated developments of antibody glycosylation evaluation in biomedical, biopharmaceutical and scientific analysis.

Native and Denaturing MS Protein Deconvolution for Biopharma: Monoclonal Antibodies and Antibody-Drug Conjugates to Polydisperse Membrane Proteins and Past.

Electrospray ionization mass spectrometry (ESI-MS) is a ubiquitously used analytical technique utilized throughout a number of departments in biopharma, starting from early analysis discovery to course of growth. Correct, environment friendly, and constant protein MS spectral deconvolution throughout a number of instrument and detector platforms (time-of-flight, Orbitrap, Fourier-transform ion cyclotron resonance) is crucial.

When proteins are ionized in the course of the ESI course of, a distribution of consecutive multiply charged ions are noticed on the m/z scale, both constructive [M + nH]n+ or detrimental [M – nH]n relying on the ionization polarity. The guide calculation of the impartial molecular weight (MW) of single proteins measured by ESI-MS is easy; nevertheless, algorithmic deconvolution is required for extra complicated protein mixtures to derive correct MWs. A number of deconvolution algorithms have developed over the previous twenty years, all of which have their benefits and downsides, when it comes to pace, user-input parameters (or ideally lack thereof), and whether or not they carry out optimally on proteins analyzed underneath denatured or native-MS and resolution situations.

 

Herein, we describe the utility of a parsimonious deconvolution algorithm (explaining the noticed spectra with a minimal variety of plenty) to course of a variety of extremely numerous biopharma related and analysis grade proteins and complexes (PEG-GCSF; an IgG1ok; IgG1- and IgG2-biotin covalent conjugates; the membrane protein complicated AqpZ; a extremely polydisperse empty MSP1D1 nanodisc and the tetradecameric chaperone protein complicated GroEL) analyzed underneath native-MS, denaturing LC-MS, and constructive and detrimental modes of ionization, utilizing a number of devices and subsequently a number of information codecs. The implementation of a comb filter and peak sharpening possibility can be demonstrated to be extremely efficient for deconvolution of extremely polydisperse and enhanced separation of a low stage lysine glycation post-translational modification (+162.1 Da), partially processed heavy chain lysine residues (+128.1 Da), and lack of N-acetylglucosamine (GlcNAc; -203.1 Da).

Monitoring of Immunoglobulin N- and O-glycosylation in Health and Disease.

Nanoscale integration of single cell biologics discovery processes utilizing optofluidic manipulation and monitoring.

The brand new and speedy development within the complexity of biologics drug discovery has been pushed by a deeper understanding of organic techniques mixed with progressive new therapeutic modalities, paving the best way to breakthrough therapies for beforehand intractable illnesses. These thrilling occasions in biomedical innovation require the event of novel applied sciences to facilitate the subtle, multifaceted, high-paced workflows essential to help trendy giant molecule drug discovery.
A high-level aspiration is a real integration of “lab-on-a-chip” strategies that vastly miniaturize cellulmical experiments might rework the pace, price, and success of a number of workstreams in biologics growth. A number of microscale bioprocess applied sciences have been established that incrementally handle these wants, but every is inflexibly designed for a really particular course of thus limiting an built-in holistic software. A extra totally built-in nanoscale strategy that comes with manipulation, tradition, analytics, and traceable digital report maintaining of hundreds of single cells in a related nanoenvironment can be a transformative know-how able to maintaining tempo with in the present day’s speedy and sophisticated drug discovery calls for.

Rat Uterus, non-pregnant Frozen Sections

RF-411 10 slides
EUR 228

Rat WS Uterus, non-pregnant Frozen Sections

RF-411-WS 10 slides
EUR 228

Mouse CD1 Uterus, non-pregnant Frozen Sections

MF-411 10 slides
EUR 228

Mouse BLC Uterus, non-pregnant Frozen Sections

MF-411-BLC 10 slides
EUR 253

Mouse C57 Uterus, non-pregnant Frozen Sections

MF-411-C57 10 slides
EUR 253

Tissue, Section, Human Tumor, Uterus (Frozen)

MBS640240-5Sections 5Sections
EUR 620

Tissue, Section, Human Tumor, Uterus (Frozen)

MBS640240-5x5Sections 5x5Sections
EUR 2570

Frozen Tissue Section - Human Tumor: Uterus

T1235274 5 slides
EUR 363

Tissue, Section, Human Adult Normal, Uterus (Frozen)

MBS640854-5Sections 5Sections
EUR 635

Tissue, Section, Human Adult Normal, Uterus (Frozen)

MBS640854-5x5Sections 5x5Sections
EUR 2640

Frozen Tissue Section - Human Adult Normal: Uterus

T1234274 5 slides
EUR 377

Rabbit Skin Frozen Sections

TF-101 10 slides
EUR 240

Rabbit Lung Frozen Sections

TF-601 10 slides
EUR 240

Rabbit Colon Frozen Sections

TF-311 10 slides
EUR 240

Rabbit Liver Frozen Sections

TF-314 10 slides
EUR 240

Rabbit Ovary Frozen Sections

TF-406 10 slides
EUR 240

Rabbit Heart Frozen Sections

TF-801 10 slides
EUR 240

Rabbit Testis Frozen Sections

TF-401 10 slides
EUR 240

Rabbit Spleen Frozen Sections

TF-701 10 slides
EUR 240

Rabbit Thymus Frozen Sections

TF-702 10 slides
EUR 240

Rabbit Kidney Frozen Sections

TF-901 10 slides
EUR 240

Rabbit Stomach Frozen Sections

TF-302 10 slides
EUR 240

Rabbit Adrenal Frozen Sections

TF-501 10 slides
EUR 240

Rabbit Bladder Frozen Sections

TF-902 10 slides
EUR 240

Rabbit Pancreas Frozen Sections

TF-313 10 slides
EUR 240

Rabbit Prostate Frozen Sections

TF-408 10 slides
EUR 240

Rabbit Esophagus Frozen Sections

TF-301 10 slides
EUR 240

Rabbit Skeletal Muscles Frozen Sections

TF-102 10 slides
EUR 240

Rabbit Brain, Cerebellum Frozen Sections

TF-202 10 slides
EUR 240

Tissue, Section, Human Adult Normal, Uterus, <=47yrs, w/ Endometrium (Frozen)

MBS640095-5Sections 5Sections
EUR 635

Tissue, Section, Human Adult Normal, Uterus, <=47yrs, w/ Endometrium (Frozen)

MBS640095-5x5Sections 5x5Sections
EUR 2640

Rabbit Small Intestine, Ileum Frozen Sections

TF-309 10 slides
EUR 240

Rabbit Small Intestine, Jejunum Frozen Sections

TF-308 10 slides
EUR 240

Rabbit Small Intestine, Duodenum Frozen Sections

TF-307 10 slides
EUR 240

Rabbit Brain, Hypothalamus/Hippocampus Frozen Sections

TF-204 10 slides
EUR 240

Rabbit Brain, Striatum/Cerebral Cortex Frozen Sections

TF-214 10 slides
EUR 240

Pig Uterus Paraffin Sections

PP-411 10 slides
EUR 240

Human Uterus Paraffin Sections

HP-411 10 slides
EUR 319

Sheep Uterus Paraffin Sections

SP-411 10 slides
EUR 240

Bovine Uterus Paraffin Sections

BP-411 10 slides
EUR 240

Equine Uterus Paraffin Sections

EP-411 10 slides
EUR 240

Hamster Uterus Paraffin Sections

AP-411 10 slides
EUR 228

MiniPig Uterus Paraffin Sections

NP-411 10 slides
EUR 307

Rabbit Tissue Frozen Sections Panel, Set of any 10 tissues

TF-010 10X4 slides
EUR 888

Guinea Pig Uterus Paraffin Sections

GP-411 10 slides
EUR 228

Rat Eye Frozen Sections

RF-106 10 slides
EUR 266

Dog Skin Frozen Sections

DF-101 10 slides
EUR 261

Dog Lung Frozen Sections

DF-601 10 slides
EUR 261

Cat Lung Frozen Sections

FF-601 10 slides
EUR 261

Pig Skin Frozen Sections

PF-101 10 slides
EUR 261

Pig Lung Frozen Sections

PF-601 10 slides
EUR 261

Rat Skin Frozen Sections

RF-101 10 slides
EUR 228

Rat Lung Frozen Sections

RF-601 10 slides
EUR 228

Dog Liver Frozen Sections

DF-314 10 slides
EUR 261

Dog Ovary Frozen Sections

DF-406 10 slides
EUR 261

Dog Heart Frozen Sections

DF-801 10 slides
EUR 261

Cat Liver Frozen Sections

FF-314 10 slides
EUR 261

Cat Ovary Frozen Sections

FF-406 10 slides
EUR 261

Cat Heart Frozen Sections

FF-801 10 slides
EUR 261

Pig Colon Frozen Sections

PF-211 10 slides
EUR 261

Pig Liver Frozen Sections

PF-314 10 slides
EUR 261

Pig Ovary Frozen Sections

PF-406 10 slides
EUR 261

Pig Heart Frozen Sections

PF-801 10 slides
EUR 261

Pig Aorta Frozen Sections

PF-807 10 slides
EUR 261

Rat Cecum Frozen Sections

RF-310 10 slides
EUR 228

Rat Colon Frozen Sections

RF-311 10 slides
EUR 228

Rat Liver Frozen Sections

RF-314 10 slides
EUR 228

Rat Ovary Frozen Sections

RF-406 10 slides
EUR 228

Rat Penis Frozen Sections

RF-416 10 slides
EUR 228

Rat Aorta Frozen Sections

RF-807 10 slides
EUR 266

Dog Tongue Frozen Sections

DF-105 10 slides
EUR 261

Dog Rectum Frozen Sections

DF-312 10 slides
EUR 261

Dog Testis Frozen Sections

DF-401 10 slides
EUR 261

Dog Spleen Frozen Sections

DF-701 10 slides
EUR 261

Dog Thymus Frozen Sections

DF-702 10 slides
EUR 261

Dog Kidney Frozen Sections

DF-901 10 slides
EUR 261

Cat Tongue Frozen Sections

FF-105 10 slides
EUR 261

Cat Rectum Frozen Sections

FF-312 10 slides
EUR 261

Cat Testis Frozen Sections

FF-401 10 slides
EUR 261

Cat Spleen Frozen Sections

FF-701 10 slides
EUR 261

Cat Thymus Frozen Sections

FF-702 10 slides
EUR 261

Cat Kidney Frozen Sections

FF-901 10 slides
EUR 261

Human Skin Frozen Sections

HF-101 10slides
EUR 240

Human Lung Frozen Sections

HF-601 10slides
EUR 240

Human Vein Frozen Sections

HF-821 10slides
EUR 240

Pig Testis Frozen Sections

PF-401 10 slides
EUR 261

Pig Pineal Frozen Sections

PF-505 10 slides
EUR 261

Pig Spleen Frozen Sections

PF-701 10 slides
EUR 261

Pig Thymus Frozen Sections

PF-702 10 slides
EUR 261

Pig Kidney Frozen Sections

PF-901 10 slides
EUR 261

Rat Tongue Frozen Sections

RF-105 10 slides
EUR 266

Rat Rectum Frozen Sections

RF-312 10 slides
EUR 228

Rat Testis Frozen Sections

RF-401 10 slides
EUR 228

Rat Vagina Frozen Sections

RF-412 10 slides
EUR 228

Rat Spleen Frozen Sections

RF-701 10 slides
EUR 228

Rat Thymus Frozen Sections

RF-702 10 slides
EUR 228

Rat Kidney Frozen Sections

RF-901 10 slides
EUR 228

Sheep Skin Frozen Sections

SF-101 10 slides
EUR 261

Sheep Lung Frozen Sections

SF-601 10 slides
EUR 261

Equine Skin Frozen Sections

EF-101 10 slides
EUR 261

Equine Lung Frozen Sections

EF-601 10 slides
EUR 261

Dog Stomach Frozen Sections

DF-302 10 slides
EUR 261

Dog Bladder Frozen Sections

DF-902 10 slides
EUR 261

Bovine Skin Frozen Sections

BF-101 10 slides
EUR 261

Bovine Lung Frozen Sections

BF-601 10 slides
EUR 261

Cat Stomach Frozen Sections

FF-302 10 slides
EUR 261

Cat Bladder Frozen Sections

FF-902 10 slides
EUR 261

Human Colon Frozen Sections

HF-311 10slides
EUR 240

Human Liver Frozen Sections

HF-314 10 slides
EUR 240

Human Ovary Frozen Sections

HF-406 10slides
EUR 240

Human Heart Frozen Sections

HF-801 10slides
EUR 240

Pig Stomach Frozen Sections

PF-302 10 slides
EUR 261

Pig Adrenal Frozen Sections

PF-501 10 slides
EUR 261

Pig Thyroid Frozen Sections

PF-503 10 slides
EUR 261

Pig Bladder Frozen Sections

PF-902 10 slides
EUR 261

Rat Oviduct Frozen Sections

RF-410 10 slides
EUR 228

Rat Adrenal Frozen Sections

RF-501 10 slides
EUR 266

Rat Thyroid Frozen Sections

RF-503 10 slides
EUR 266

Rat Trachea Frozen Sections

RF-602 10 slides
EUR 266

Rat Bladder Frozen Sections

RF-902 10 slides
EUR 228

Rat Urethra Frozen Sections

RF-903 10 slides
EUR 228

Sheep Colon Frozen Sections

SF-311 10 slides
EUR 261

Sheep Liver Frozen Sections

SF-314 10 slides
EUR 261

Sheep Ovary Frozen Sections

SF-406 10 slides
EUR 261

Sheep Heart Frozen Sections

SF-801 10 slides
EUR 261

BOVINE, UTERUS, FROZEN

8620861 1EA
EUR 67.09
The current introduction of optical manipulation of cells utilizing light-induced electrokinetics with micro- and nanoscale cell tradition is poised to revolutionize each elementary and utilized organic analysis. On this overview, we summarize the present cutting-edge for optical manipulation methods and talk about rising organic functions of this know-how. Particularly, we give attention to promising prospects for drug discovery workflows, together with antibody discovery, bioassay growth, antibody engineering, and cell line growth, that are enabled by the automation and industrialization of an built-in optoelectronic single-cell manipulation and tradition platform. Continued growth of such platforms will likely be properly positioned to beat most of the challenges at the moment related to fragmented, low-throughput bioprocess workflows in biopharma and life science analysis.

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