Protein N- and O- glycosylation are well-known co- and post-translational modifications of immunoglobulins. Antibody glycosylation on the Fab and Fc portion is understood to affect antigen binding and effector features, respectively. To check associations between antibody glycosylation profiles and (patho)physiological states in addition to antibody performance, superior applied sciences and strategies are required. In-depth structural characterization of antibody glycosylation often depends on the separation and tandem MS evaluation of launched glycans. Protein- and site-specific data, alternatively, could also be obtained by the MS evaluation of glycopeptides.
With the event of high-resolution mass spectrometers, antibody glycosylation evaluation on the intact or middle-up stage has gained extra curiosity, offering an built-in view of various post-translational modifications (together with glycosylation). Alongside the in-depth strategies, there’s additionally nice curiosity in sturdy, high-throughput methods for routine glycosylation profiling in biopharma and scientific laboratories. With an emphasis on IgG Fc glycosylation, a number of extremely sturdy separation-based methods are employed for this goal. On this overview, we describe current advances in mass spectrometric strategies, separation methods and orthogonal approaches for the characterization of immunoglobulin glycosylation in numerous settings. We put emphasis on the present standing and anticipated developments of antibody glycosylation evaluation in biomedical, biopharmaceutical and scientific analysis.
Native and Denaturing MS Protein Deconvolution for Biopharma: Monoclonal Antibodies and Antibody-Drug Conjugates to Polydisperse Membrane Proteins and Past.
Electrospray ionization mass spectrometry (ESI-MS) is a ubiquitously used analytical technique utilized throughout a number of departments in biopharma, starting from early analysis discovery to course of growth. Correct, environment friendly, and constant protein MS spectral deconvolution throughout a number of instrument and detector platforms (time-of-flight, Orbitrap, Fourier-transform ion cyclotron resonance) is crucial.
When proteins are ionized in the course of the ESI course of, a distribution of consecutive multiply charged ions are noticed on the m/z scale, both constructive [M + nH]n+ or detrimental [M – nH]n– relying on the ionization polarity. The guide calculation of the impartial molecular weight (MW) of single proteins measured by ESI-MS is easy; nevertheless, algorithmic deconvolution is required for extra complicated protein mixtures to derive correct MWs. A number of deconvolution algorithms have developed over the previous twenty years, all of which have their benefits and downsides, when it comes to pace, user-input parameters (or ideally lack thereof), and whether or not they carry out optimally on proteins analyzed underneath denatured or native-MS and resolution situations.
Herein, we describe the utility of a parsimonious deconvolution algorithm (explaining the noticed spectra with a minimal variety of plenty) to course of a variety of extremely numerous biopharma related and analysis grade proteins and complexes (PEG-GCSF; an IgG1ok; IgG1- and IgG2-biotin covalent conjugates; the membrane protein complicated AqpZ; a extremely polydisperse empty MSP1D1 nanodisc and the tetradecameric chaperone protein complicated GroEL) analyzed underneath native-MS, denaturing LC-MS, and constructive and detrimental modes of ionization, utilizing a number of devices and subsequently a number of information codecs. The implementation of a comb filter and peak sharpening possibility can be demonstrated to be extremely efficient for deconvolution of extremely polydisperse and enhanced separation of a low stage lysine glycation post-translational modification (+162.1 Da), partially processed heavy chain lysine residues (+128.1 Da), and lack of N-acetylglucosamine (GlcNAc; -203.1 Da).
Nanoscale integration of single cell biologics discovery processes utilizing optofluidic manipulation and monitoring.
The brand new and speedy development within the complexity of biologics drug discovery has been pushed by a deeper understanding of organic techniques mixed with progressive new therapeutic modalities, paving the best way to breakthrough therapies for beforehand intractable illnesses. These thrilling occasions in biomedical innovation require the event of novel applied sciences to facilitate the subtle, multifaceted, high-paced workflows essential to help trendy giant molecule drug discovery.
A high-level aspiration is a real integration of “lab-on-a-chip” strategies that vastly miniaturize cellulmical experiments might rework the pace, price, and success of a number of workstreams in biologics growth. A number of microscale bioprocess applied sciences have been established that incrementally handle these wants, but every is inflexibly designed for a really particular course of thus limiting an built-in holistic software. A extra totally built-in nanoscale strategy that comes with manipulation, tradition, analytics, and traceable digital report maintaining of hundreds of single cells in a related nanoenvironment can be a transformative know-how able to maintaining tempo with in the present day’s speedy and sophisticated drug discovery calls for.
 Rat Uterus, non-pregnant Frozen Sections |
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RF-411 |
Zyagen |
10 slides |
EUR 228 |
 Rat WS Uterus, non-pregnant Frozen Sections |
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RF-411-WS |
Zyagen |
10 slides |
EUR 228 |
 Mouse CD1 Uterus, non-pregnant Frozen Sections |
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MF-411 |
Zyagen |
10 slides |
EUR 228 |
 Mouse BLC Uterus, non-pregnant Frozen Sections |
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MF-411-BLC |
Zyagen |
10 slides |
EUR 253 |
 Mouse C57 Uterus, non-pregnant Frozen Sections |
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MF-411-C57 |
Zyagen |
10 slides |
EUR 253 |
) Tissue, Section, Human Tumor, Uterus (Frozen) |
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MBS640240-5Sections |
MyBiosource |
5Sections |
EUR 620 |
Tissue, Section, Human Tumor, Uterus (Frozen) |
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MBS640240-5x5Sections |
MyBiosource |
5x5Sections |
EUR 2570 |
 Frozen Tissue Section - Human Tumor: Uterus |
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T1235274 |
Biochain |
5 slides |
EUR 363 |
) Tissue, Section, Human Adult Normal, Uterus (Frozen) |
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MBS640854-5Sections |
MyBiosource |
5Sections |
EUR 635 |
Tissue, Section, Human Adult Normal, Uterus (Frozen) |
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MBS640854-5x5Sections |
MyBiosource |
5x5Sections |
EUR 2640 |
 Frozen Tissue Section - Human Adult Normal: Uterus |
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T1234274 |
Biochain |
5 slides |
EUR 377 |
 Rabbit Skin Frozen Sections |
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TF-101 |
Zyagen |
10 slides |
EUR 240 |
 Rabbit Lung Frozen Sections |
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TF-601 |
Zyagen |
10 slides |
EUR 240 |
 Rabbit Colon Frozen Sections |
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TF-311 |
Zyagen |
10 slides |
EUR 240 |
 Rabbit Liver Frozen Sections |
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TF-314 |
Zyagen |
10 slides |
EUR 240 |
 Rabbit Ovary Frozen Sections |
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TF-406 |
Zyagen |
10 slides |
EUR 240 |
 Rabbit Heart Frozen Sections |
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TF-801 |
Zyagen |
10 slides |
EUR 240 |
 Rabbit Testis Frozen Sections |
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TF-401 |
Zyagen |
10 slides |
EUR 240 |
 Rabbit Spleen Frozen Sections |
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TF-701 |
Zyagen |
10 slides |
EUR 240 |
 Rabbit Thymus Frozen Sections |
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TF-702 |
Zyagen |
10 slides |
EUR 240 |
 Rabbit Kidney Frozen Sections |
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TF-901 |
Zyagen |
10 slides |
EUR 240 |
 Rabbit Stomach Frozen Sections |
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TF-302 |
Zyagen |
10 slides |
EUR 240 |
 Rabbit Adrenal Frozen Sections |
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TF-501 |
Zyagen |
10 slides |
EUR 240 |
 Rabbit Bladder Frozen Sections |
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TF-902 |
Zyagen |
10 slides |
EUR 240 |
 Rabbit Pancreas Frozen Sections |
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TF-313 |
Zyagen |
10 slides |
EUR 240 |
 Rabbit Prostate Frozen Sections |
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TF-408 |
Zyagen |
10 slides |
EUR 240 |
 Rabbit Esophagus Frozen Sections |
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TF-301 |
Zyagen |
10 slides |
EUR 240 |
 Rabbit Skeletal Muscles Frozen Sections |
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TF-102 |
Zyagen |
10 slides |
EUR 240 |
 Rabbit Brain, Cerebellum Frozen Sections |
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TF-202 |
Zyagen |
10 slides |
EUR 240 |
) Tissue, Section, Human Adult Normal, Uterus, <=47yrs, w/ Endometrium (Frozen) |
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MBS640095-5Sections |
MyBiosource |
5Sections |
EUR 635 |
Tissue, Section, Human Adult Normal, Uterus, <=47yrs, w/ Endometrium (Frozen) |
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MBS640095-5x5Sections |
MyBiosource |
5x5Sections |
EUR 2640 |
 Rabbit Small Intestine, Ileum Frozen Sections |
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TF-309 |
Zyagen |
10 slides |
EUR 240 |
 Rabbit Small Intestine, Jejunum Frozen Sections |
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TF-308 |
Zyagen |
10 slides |
EUR 240 |
 Rabbit Small Intestine, Duodenum Frozen Sections |
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TF-307 |
Zyagen |
10 slides |
EUR 240 |
 Rabbit Brain, Hypothalamus/Hippocampus Frozen Sections |
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TF-204 |
Zyagen |
10 slides |
EUR 240 |
 Rabbit Brain, Striatum/Cerebral Cortex Frozen Sections |
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TF-214 |
Zyagen |
10 slides |
EUR 240 |
 Pig Uterus Paraffin Sections |
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PP-411 |
Zyagen |
10 slides |
EUR 240 |
 Human Uterus Paraffin Sections |
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HP-411 |
Zyagen |
10 slides |
EUR 319 |
 Sheep Uterus Paraffin Sections |
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SP-411 |
Zyagen |
10 slides |
EUR 240 |
 Bovine Uterus Paraffin Sections |
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BP-411 |
Zyagen |
10 slides |
EUR 240 |
 Equine Uterus Paraffin Sections |
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EP-411 |
Zyagen |
10 slides |
EUR 240 |
 Hamster Uterus Paraffin Sections |
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AP-411 |
Zyagen |
10 slides |
EUR 228 |
 MiniPig Uterus Paraffin Sections |
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NP-411 |
Zyagen |
10 slides |
EUR 307 |
 Rabbit Tissue Frozen Sections Panel, Set of any 10 tissues |
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TF-010 |
Zyagen |
10X4 slides |
EUR 888 |
 Rat Eye Frozen Sections |
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RF-106 |
Zyagen |
10 slides |
EUR 266 |
 Guinea Pig Uterus Paraffin Sections |
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GP-411 |
Zyagen |
10 slides |
EUR 228 |
 Dog Skin Frozen Sections |
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DF-101 |
Zyagen |
10 slides |
EUR 261 |
 Dog Lung Frozen Sections |
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DF-601 |
Zyagen |
10 slides |
EUR 261 |
 Cat Lung Frozen Sections |
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FF-601 |
Zyagen |
10 slides |
EUR 261 |
 Pig Skin Frozen Sections |
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PF-101 |
Zyagen |
10 slides |
EUR 261 |
 Pig Lung Frozen Sections |
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PF-601 |
Zyagen |
10 slides |
EUR 261 |
 Rat Skin Frozen Sections |
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RF-101 |
Zyagen |
10 slides |
EUR 228 |
 Rat Lung Frozen Sections |
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RF-601 |
Zyagen |
10 slides |
EUR 228 |
 Dog Liver Frozen Sections |
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DF-314 |
Zyagen |
10 slides |
EUR 261 |
 Dog Ovary Frozen Sections |
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DF-406 |
Zyagen |
10 slides |
EUR 261 |
 Dog Heart Frozen Sections |
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DF-801 |
Zyagen |
10 slides |
EUR 261 |
 Cat Liver Frozen Sections |
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FF-314 |
Zyagen |
10 slides |
EUR 261 |
 Cat Ovary Frozen Sections |
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FF-406 |
Zyagen |
10 slides |
EUR 261 |
 Cat Heart Frozen Sections |
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FF-801 |
Zyagen |
10 slides |
EUR 261 |
 Pig Colon Frozen Sections |
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PF-211 |
Zyagen |
10 slides |
EUR 261 |
 Pig Liver Frozen Sections |
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PF-314 |
Zyagen |
10 slides |
EUR 261 |
 Pig Ovary Frozen Sections |
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PF-406 |
Zyagen |
10 slides |
EUR 261 |
 Pig Heart Frozen Sections |
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PF-801 |
Zyagen |
10 slides |
EUR 261 |
 Pig Aorta Frozen Sections |
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PF-807 |
Zyagen |
10 slides |
EUR 261 |
 Rat Cecum Frozen Sections |
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RF-310 |
Zyagen |
10 slides |
EUR 228 |
 Rat Colon Frozen Sections |
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RF-311 |
Zyagen |
10 slides |
EUR 228 |
 Rat Liver Frozen Sections |
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RF-314 |
Zyagen |
10 slides |
EUR 228 |
 Rat Ovary Frozen Sections |
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RF-406 |
Zyagen |
10 slides |
EUR 228 |
 Rat Penis Frozen Sections |
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RF-416 |
Zyagen |
10 slides |
EUR 228 |
 Rat Aorta Frozen Sections |
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RF-807 |
Zyagen |
10 slides |
EUR 266 |
 Dog Tongue Frozen Sections |
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DF-105 |
Zyagen |
10 slides |
EUR 261 |
 Dog Rectum Frozen Sections |
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DF-312 |
Zyagen |
10 slides |
EUR 261 |
 Dog Testis Frozen Sections |
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DF-401 |
Zyagen |
10 slides |
EUR 261 |
 Dog Spleen Frozen Sections |
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DF-701 |
Zyagen |
10 slides |
EUR 261 |
 Dog Thymus Frozen Sections |
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DF-702 |
Zyagen |
10 slides |
EUR 261 |
 Dog Kidney Frozen Sections |
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DF-901 |
Zyagen |
10 slides |
EUR 261 |
 Cat Tongue Frozen Sections |
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FF-105 |
Zyagen |
10 slides |
EUR 261 |
 Cat Rectum Frozen Sections |
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FF-312 |
Zyagen |
10 slides |
EUR 261 |
 Cat Testis Frozen Sections |
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FF-401 |
Zyagen |
10 slides |
EUR 261 |
 Cat Spleen Frozen Sections |
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FF-701 |
Zyagen |
10 slides |
EUR 261 |
 Cat Thymus Frozen Sections |
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FF-702 |
Zyagen |
10 slides |
EUR 261 |
 Cat Kidney Frozen Sections |
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FF-901 |
Zyagen |
10 slides |
EUR 261 |
 Human Skin Frozen Sections |
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HF-101 |
Zyagen |
10slides |
EUR 240 |
 Human Lung Frozen Sections |
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HF-601 |
Zyagen |
10slides |
EUR 240 |
 Human Vein Frozen Sections |
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HF-821 |
Zyagen |
10slides |
EUR 240 |
 Pig Testis Frozen Sections |
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PF-401 |
Zyagen |
10 slides |
EUR 261 |
 Pig Pineal Frozen Sections |
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PF-505 |
Zyagen |
10 slides |
EUR 261 |
 Pig Spleen Frozen Sections |
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PF-701 |
Zyagen |
10 slides |
EUR 261 |
 Pig Thymus Frozen Sections |
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PF-702 |
Zyagen |
10 slides |
EUR 261 |
 Pig Kidney Frozen Sections |
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PF-901 |
Zyagen |
10 slides |
EUR 261 |
 Rat Tongue Frozen Sections |
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RF-105 |
Zyagen |
10 slides |
EUR 266 |
 Rat Rectum Frozen Sections |
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RF-312 |
Zyagen |
10 slides |
EUR 228 |
 Rat Testis Frozen Sections |
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RF-401 |
Zyagen |
10 slides |
EUR 228 |
 Rat Vagina Frozen Sections |
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RF-412 |
Zyagen |
10 slides |
EUR 228 |
 Rat Spleen Frozen Sections |
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RF-701 |
Zyagen |
10 slides |
EUR 228 |
 Rat Thymus Frozen Sections |
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RF-702 |
Zyagen |
10 slides |
EUR 228 |
 Rat Kidney Frozen Sections |
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RF-901 |
Zyagen |
10 slides |
EUR 228 |
 Sheep Skin Frozen Sections |
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SF-101 |
Zyagen |
10 slides |
EUR 261 |
 Sheep Lung Frozen Sections |
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SF-601 |
Zyagen |
10 slides |
EUR 261 |
 Equine Skin Frozen Sections |
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EF-101 |
Zyagen |
10 slides |
EUR 261 |
 Equine Lung Frozen Sections |
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EF-601 |
Zyagen |
10 slides |
EUR 261 |
 Dog Stomach Frozen Sections |
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DF-302 |
Zyagen |
10 slides |
EUR 261 |
 Dog Bladder Frozen Sections |
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DF-902 |
Zyagen |
10 slides |
EUR 261 |
 Bovine Skin Frozen Sections |
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BF-101 |
Zyagen |
10 slides |
EUR 261 |
 Bovine Lung Frozen Sections |
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BF-601 |
Zyagen |
10 slides |
EUR 261 |
 Cat Stomach Frozen Sections |
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FF-302 |
Zyagen |
10 slides |
EUR 261 |
 Cat Bladder Frozen Sections |
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FF-902 |
Zyagen |
10 slides |
EUR 261 |
 Human Colon Frozen Sections |
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HF-311 |
Zyagen |
10slides |
EUR 240 |
 Human Liver Frozen Sections |
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HF-314 |
Zyagen |
10 slides |
EUR 240 |
 Human Ovary Frozen Sections |
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HF-406 |
Zyagen |
10slides |
EUR 240 |
 Human Heart Frozen Sections |
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HF-801 |
Zyagen |
10slides |
EUR 240 |
 Pig Stomach Frozen Sections |
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PF-302 |
Zyagen |
10 slides |
EUR 261 |
 Pig Adrenal Frozen Sections |
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PF-501 |
Zyagen |
10 slides |
EUR 261 |
 Pig Thyroid Frozen Sections |
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PF-503 |
Zyagen |
10 slides |
EUR 261 |
 Pig Bladder Frozen Sections |
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PF-902 |
Zyagen |
10 slides |
EUR 261 |
 Rat Oviduct Frozen Sections |
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RF-410 |
Zyagen |
10 slides |
EUR 228 |
 Rat Adrenal Frozen Sections |
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RF-501 |
Zyagen |
10 slides |
EUR 266 |
 Rat Thyroid Frozen Sections |
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RF-503 |
Zyagen |
10 slides |
EUR 266 |
 Rat Trachea Frozen Sections |
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RF-602 |
Zyagen |
10 slides |
EUR 266 |
 Rat Bladder Frozen Sections |
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RF-902 |
Zyagen |
10 slides |
EUR 228 |
 Rat Urethra Frozen Sections |
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RF-903 |
Zyagen |
10 slides |
EUR 228 |
 Sheep Colon Frozen Sections |
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SF-311 |
Zyagen |
10 slides |
EUR 261 |
 Sheep Liver Frozen Sections |
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SF-314 |
Zyagen |
10 slides |
EUR 261 |
 Sheep Ovary Frozen Sections |
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SF-406 |
Zyagen |
10 slides |
EUR 261 |
 Sheep Heart Frozen Sections |
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SF-801 |
Zyagen |
10 slides |
EUR 261 |
The current introduction of optical manipulation of cells utilizing light-induced electrokinetics with micro- and nanoscale cell tradition is poised to revolutionize each elementary and utilized organic analysis. On this overview, we summarize the present cutting-edge for optical manipulation methods and talk about rising organic functions of this know-how. Particularly, we give attention to promising prospects for drug discovery workflows, together with antibody discovery, bioassay growth, antibody engineering, and cell line growth, that are enabled by the automation and industrialization of an built-in optoelectronic single-cell manipulation and tradition platform. Continued growth of such platforms will likely be properly positioned to beat most of the challenges at the moment related to fragmented, low-throughput bioprocess workflows in biopharma and life science analysis.
