Protein N- and O- glycosylation are well-known co- and post-translational modifications of immunoglobulins. Antibody glycosylation on the Fab and Fc portion is understood to affect antigen binding and effector features, respectively. To check associations between antibody glycosylation profiles and (patho)physiological states in addition to antibody performance, superior applied sciences and strategies are required. In-depth structural characterization of antibody glycosylation often depends on the separation and tandem MS evaluation of launched glycans. Protein- and site-specific data, alternatively, could also be obtained by the MS evaluation of glycopeptides.
With the event of high-resolution mass spectrometers, antibody glycosylation evaluation on the intact or middle-up stage has gained extra curiosity, offering an built-in view of various post-translational modifications (together with glycosylation). Alongside the in-depth strategies, there’s additionally nice curiosity in sturdy, high-throughput methods for routine glycosylation profiling in biopharma and scientific laboratories. With an emphasis on IgG Fc glycosylation, a number of extremely sturdy separation-based methods are employed for this goal. On this overview, we describe current advances in mass spectrometric strategies, separation methods and orthogonal approaches for the characterization of immunoglobulin glycosylation in numerous settings. We put emphasis on the present standing and anticipated developments of antibody glycosylation evaluation in biomedical, biopharmaceutical and scientific analysis.
Native and Denaturing MS Protein Deconvolution for Biopharma: Monoclonal Antibodies and Antibody-Drug Conjugates to Polydisperse Membrane Proteins and Past.
Electrospray ionization mass spectrometry (ESI-MS) is a ubiquitously used analytical technique utilized throughout a number of departments in biopharma, starting from early analysis discovery to course of growth. Correct, environment friendly, and constant protein MS spectral deconvolution throughout a number of instrument and detector platforms (time-of-flight, Orbitrap, Fourier-transform ion cyclotron resonance) is crucial.
When proteins are ionized in the course of the ESI course of, a distribution of consecutive multiply charged ions are noticed on the m/z scale, both constructive [M + nH]n+ or detrimental [M – nH]n– relying on the ionization polarity. The guide calculation of the impartial molecular weight (MW) of single proteins measured by ESI-MS is easy; nevertheless, algorithmic deconvolution is required for extra complicated protein mixtures to derive correct MWs. A number of deconvolution algorithms have developed over the previous twenty years, all of which have their benefits and downsides, when it comes to pace, user-input parameters (or ideally lack thereof), and whether or not they carry out optimally on proteins analyzed underneath denatured or native-MS and resolution situations.
Herein, we describe the utility of a parsimonious deconvolution algorithm (explaining the noticed spectra with a minimal variety of plenty) to course of a variety of extremely numerous biopharma related and analysis grade proteins and complexes (PEG-GCSF; an IgG1ok; IgG1- and IgG2-biotin covalent conjugates; the membrane protein complicated AqpZ; a extremely polydisperse empty MSP1D1 nanodisc and the tetradecameric chaperone protein complicated GroEL) analyzed underneath native-MS, denaturing LC-MS, and constructive and detrimental modes of ionization, utilizing a number of devices and subsequently a number of information codecs. The implementation of a comb filter and peak sharpening possibility can be demonstrated to be extremely efficient for deconvolution of extremely polydisperse and enhanced separation of a low stage lysine glycation post-translational modification (+162.1 Da), partially processed heavy chain lysine residues (+128.1 Da), and lack of N-acetylglucosamine (GlcNAc; -203.1 Da).
Nanoscale integration of single cell biologics discovery processes utilizing optofluidic manipulation and monitoring.
The brand new and speedy development within the complexity of biologics drug discovery has been pushed by a deeper understanding of organic techniques mixed with progressive new therapeutic modalities, paving the best way to breakthrough therapies for beforehand intractable illnesses. These thrilling occasions in biomedical innovation require the event of novel applied sciences to facilitate the subtle, multifaceted, high-paced workflows essential to help trendy giant molecule drug discovery.
A high-level aspiration is a real integration of “lab-on-a-chip” strategies that vastly miniaturize cellulmical experiments might rework the pace, price, and success of a number of workstreams in biologics growth. A number of microscale bioprocess applied sciences have been established that incrementally handle these wants, but every is inflexibly designed for a really particular course of thus limiting an built-in holistic software. A extra totally built-in nanoscale strategy that comes with manipulation, tradition, analytics, and traceable digital report maintaining of hundreds of single cells in a related nanoenvironment can be a transformative know-how able to maintaining tempo with in the present day’s speedy and sophisticated drug discovery calls for.
 Frozen Tissue Section - Lupus: Kidney |
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EUR 587 |
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EUR 330 |
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 (5 slides/pack)) Rat Normal Uterus (single section per slide) (5 slides/pack) |
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EUR 150 |
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EUR 330 |
 (5 slides/pack)) Normal Human Uterus (single section per slide) (5 slides/pack) |
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HuFPT096 |
TissueArray |
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EUR 150 |
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EUR 1060 |
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EUR 524 |
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EUR 1060 |
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EUR 646.8 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
 Paraffin Tissue Section - Human Uterus Cervix Tumor, HPV negative |
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T2235275HPV- |
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EUR 646.8 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
Paraffin Tissue Section - Human Uterus Cervix Tumor, HPV negative |
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 (5 slides/pack)) Cynomolgus Monkey: Uterus (single section per slide) (5 slides/pack) |
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T1236052Dem |
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EUR 343 |
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T1236051Dep |
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T1236052Dep |
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EUR 883 |
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T1234106 |
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EUR 238 |
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EUR 343 |
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EUR 758 |
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EUR 475 |
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EUR 794 |
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 Rabbit UTERUS Y 25 EA* |
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41253-2 |
Pel-Freez |
25 EA |
EUR 382.07 |
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5 slides |
EUR 883 |
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EUR 475 |
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T1234226 |
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T1236108Dia |
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EUR 662 |
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EUR 475 |
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5 slides |
EUR 475 |
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EUR 475 |
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5 slides |
EUR 343 |
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5 slides |
EUR 475 |
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EUR 475 |
The current introduction of optical manipulation of cells utilizing light-induced electrokinetics with micro- and nanoscale cell tradition is poised to revolutionize each elementary and utilized organic analysis. On this overview, we summarize the present cutting-edge for optical manipulation methods and talk about rising organic functions of this know-how. Particularly, we give attention to promising prospects for drug discovery workflows, together with antibody discovery, bioassay growth, antibody engineering, and cell line growth, that are enabled by the automation and industrialization of an built-in optoelectronic single-cell manipulation and tradition platform. Continued growth of such platforms will likely be properly positioned to beat most of the challenges at the moment related to fragmented, low-throughput bioprocess workflows in biopharma and life science analysis.
