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Synthetic biology industry: data-driven design is creating new opportunities in biotechnology

Synthetic biology industry: data-driven design is creating new opportunities in biotechnology

Artificial biology is a quickly rising interdisciplinary analysis discipline that’s primarily constructed upon foundational advances in molecular biology mixed with engineering design. The sector considers dwelling techniques as programmable on the genetic stage and has been outlined by the event of latest platform applied sciences. This has spurned a fast progress in start-up corporations and the brand new artificial biology {industry} is rising quickly, with start-up corporations receiving ∼$6.1B funding since 2015 and a world artificial biology market worth estimated to be $14B by 2026.

Most of the new start-ups will be grouped inside a multi-layer ‘expertise stack’. The ‘stack’ includes quite a few expertise layers which collectively will be utilized to a variety of latest biotechnology purposes like shopper biotechnology merchandise and dwelling therapies. The ‘stack’ additionally allows new business alternatives and worth chains just like the software program design and manufacturing revolution of the 20th century. Nonetheless, the artificial biology {industry} is at a vital level, because it now requires recognisable business successes to ensure that the {industry} to broaden and scale, when it comes to funding and firms. Nonetheless, such growth might instantly problem the ethos of artificial biology, when it comes to open expertise sharing and democratisation, which may unintentionally result in multi-national firms and expertise monopolies just like the prevailing biotechnology/biopharma {industry}.

Course of Optimization and Switch of Freeze-drying in Nested Vial Programs

Scale-up and switch of freeze-drying processes is a vital problem in biopharma {industry}. With the success of small batch processing traces using rack vial holding techniques, additional detailed information about freeze-drying cycles and their scale-up for vials in a rack is required. Subsequently, product temperature (TP) profiles in addition to Kv values of vials nested in a Polyetheretherketon (PEEK) rack have been in comparison with these of vials positioned in a generally used chrome steel tray.
Moreover, each setups have been challenged with various fill quantity and partially versus absolutely loaded rack. Moreover, a course of developed for rack was in comparison with a tray freeze-drying cycle. Freeze-drying in vials positioned within the rack is markedly sooner for middle vials and extra homogeneous in comparison with vials in bulk tray setting, as indicated by TP and Kv values. Because of the extra homogeneous drying the rack is extra versatile relating to variation of the fill quantity. The important thing level for the switch of a freeze-drying cycle from rack to tray is to think about the upper sublimation charges within the rack by adapting chamber stress or shelf temperature for the tray. Moreover, switch from one rack per shelf in a laboratory freeze-dryer to pilot scale with 4 racks per shelf was profitable. Thus, understanding of the method in rack and tray setup was enhanced to make sure environment friendly scale-up and switch of freeze-drying processes.
Glycosylation impacts the properties of biologics; thus regulatory our bodies categorized it as crucial high quality attribute and drive biopharma {industry} to seize and management it all through all phases, from R&D until finish of product lifetime. The shift from originators to biosimilars additional will increase significance and extent of glycoanalysis, which thus will increase the necessity for expertise platforms enabling dependable high-throughput and in-depth glycan evaluation. On this chapter, we are going to first summarize on established glycoanalytical strategies based mostly on liquid chromatography specializing in hydrophilic interplay chromatography, capillary electrophoresis specializing in multiplexed capillary gel electrophoresis, and mass spectrometry specializing in matrix-assisted laser desorption; we are going to then spotlight two rising applied sciences based mostly on porous graphitized carbon liquid chromatography and on ion-mobility mass spectrometry as each are extremely promising instruments to ship an extra stage of data for in-depth glycan evaluation;
moreover we elaborate on the benefits and challenges of various glycoanalytical applied sciences and their complementarity; lastly, we briefly overview purposes thereof to biopharmaceutical merchandise. This chapter gives an outline of present state-of-the-art analytical approaches for glycan characterization of biopharmaceuticals that may be employed to seize glycoprotein heterogeneity in a biopharmaceutical context.
Synthetic biology industry: data-driven design is creating new opportunities in biotechnology

Enhancing goal evaluation in biomedical analysis: the GOT-IT suggestions

Educational analysis performs a key function in figuring out new drug targets, together with understanding goal biology and hyperlinks between targets and illness states. To result in new medication, nonetheless, analysis should progress from purely educational exploration to the initiation of efforts to determine and check a drug candidate in medical trials, that are sometimes performed by the biopharma {industry}. This transition will be facilitated by a well timed deal with goal evaluation points similar to target-related issues of safety, druggability and assayability, in addition to the potential for goal modulation to realize differentiation from established therapies.

ASAH2 Rabbit pAb

A7985-200ul 200 ul
EUR 459

ASAH2 Rabbit pAb

A7985-20ul 20 ul
EUR 183

ASAH2 Rabbit pAb

A7985-50ul 50 ul
EUR 223

ASAH2 Antibody

24731-100ul 100ul
EUR 390

ASAH2 Antibody

36240-100ul 100ul
EUR 252

ASAH2 Antibody

1-CSB-PA076840
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100

ASAH2 Antibody

1-CSB-PA133826
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100

ASAH2 Antibody

1-CSB-PA002170EA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:500, IF:1:50-1:500

ASAH2 Antibody

3828-100
EUR 316

ASAH2 Antibody

3828-30T
EUR 146

ASAH2 antibody

70R-12124 100 ug
EUR 403
Description: Rabbit polyclonal ASAH2 antibody

ASAH2 Antibody

4743-002mg 0.02 mg
EUR 171.82
Description: ASAH2 Antibody: Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH2 is a neutral ceramidase and key regulator of sphingolipid signaling metabolites at the cell surface, catalyzing the hydrolysis of the N-acyl linkage of ceramide at an optimal pH of 6.5-8.5. ASAH2 is a type II integral membrane protein that can be cleaved to yield a soluble secreted protein and acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger. ASAH2 is ubiquitously expressed primarily expressed with higher levels in the intestine, kidney, skeletal muscle and heart. Recent studies indicate that ASAH2 encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract.

ASAH2 Antibody

4743-01mg 0.1 mg
EUR 436.42
Description: ASAH2 Antibody: Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH2 is a neutral ceramidase and key regulator of sphingolipid signaling metabolites at the cell surface, catalyzing the hydrolysis of the N-acyl linkage of ceramide at an optimal pH of 6.5-8.5. ASAH2 is a type II integral membrane protein that can be cleaved to yield a soluble secreted protein and acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger. ASAH2 is ubiquitously expressed primarily expressed with higher levels in the intestine, kidney, skeletal muscle and heart. Recent studies indicate that ASAH2 encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract.

ASAH2 Polyclonal Antibody, HRP Conjugated

A62107 100 µg
EUR 570.55
Description: reagents widely cited

ASAH2 Polyclonal Antibody, FITC Conjugated

A62108 100 µg
EUR 570.55
Description: Ask the seller for details

ASAH2 Polyclonal Antibody, Biotin Conjugated

A62109 100 µg
EUR 570.55
Description: The best epigenetics products

Anti-ASAH2 antibody

STJ110292 100 µl
EUR 277

Anti-ASAH2 antibody

STJ114470 100 µl
EUR 277

ASAH2 Conjugated Antibody

C36240 100ul
EUR 397

ASAH2 siRNA

20-abx900469
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

ASAH2 siRNA

20-abx908269
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

ASAH2 siRNA

20-abx908270
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

ASAH2 Peptide

4743P 0.05 mg
EUR 164.75
Description: (CT) ASAH2 peptide

ASAH2 Antibody, HRP conjugated

1-CSB-PA002170EB01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

ASAH2 Antibody, FITC conjugated

1-CSB-PA002170EC01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

ASAH2 Antibody, Biotin conjugated

1-CSB-PA002170ED01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

ASAH2 cloning plasmid

CSB-CL865115HU-10ug 10ug
EUR 474
Description: A cloning plasmid for the ASAH2 gene.

ASAH2 Blocking Peptide

3828BP-50
EUR 153

ASAH2 Blocking Peptide

33R-10941 50 ug
EUR 191
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of ASAH2 antibody, catalog no. 70R-12124

Mouse ASAH2 shRNA Plasmid

20-abx974501
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Rat ASAH2 shRNA Plasmid

20-abx987160
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Human ASAH2 shRNA Plasmid

20-abx961153
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

ASAH2 Recombinant Protein (Mouse)

RP117410 100 ug Ask for price

ASAH2 Recombinant Protein (Rat)

RP191108 100 ug Ask for price

ASAH2 Recombinant Protein (Human)

RP036760 100 ug Ask for price

N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody

20-abx303129
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody

20-abx007107
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody

abx025039-400ul 400 ul
EUR 523

N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody

abx025039-80l 80 µl
EUR 286

N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody

20-abx214010
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody

20-abx214011
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody

20-abx177710
  • EUR 1205.00
  • EUR 578.00
  • 1 mg
  • 200 ug

N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody

20-abx173712
  • EUR 857.00
  • EUR 439.00
  • 1 mg
  • 200 ug

N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody (HRP)

20-abx303130
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody (FITC)

20-abx303131
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody (Biotin)

20-abx303132
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

ASAH2 ELISA Kit (Human) (OKCD01831)

OKCD01831 96 Wells
EUR 831
Description: Description of target: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.31 ng/mL

ASAH2 ORF Vector (Human) (pORF)

ORF012254 1.0 ug DNA
EUR 354

Asah2 ORF Vector (Rat) (pORF)

ORF063704 1.0 ug DNA
EUR 506

Asah2 ORF Vector (Mouse) (pORF)

ORF039138 1.0 ug DNA
EUR 506

Asah2 sgRNA CRISPR Lentivector set (Mouse)

K3499601 3 x 1.0 ug
EUR 339

ASAH2 sgRNA CRISPR Lentivector set (Human)

K0130501 3 x 1.0 ug
EUR 339

Asah2 sgRNA CRISPR Lentivector set (Rat)

K7087001 3 x 1.0 ug
EUR 339

Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) Protein

20-abx654471
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Asah2 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3499602 1.0 ug DNA
EUR 154

Asah2 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3499603 1.0 ug DNA
EUR 154

Asah2 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3499604 1.0 ug DNA
EUR 154

ASAH2 sgRNA CRISPR Lentivector (Human) (Target 1)

K0130502 1.0 ug DNA
EUR 154

ASAH2 sgRNA CRISPR Lentivector (Human) (Target 2)

K0130503 1.0 ug DNA
EUR 154

ASAH2 sgRNA CRISPR Lentivector (Human) (Target 3)

K0130504 1.0 ug DNA
EUR 154

Mouse ASAH2 (Neutral ceramidase) ELISA Kit (CUSTOM)

ELI-34287m 96 Tests
EUR 865

Human ASAH2 (Neutral ceramidase) ELISA Kit (CUSTOM)

ELI-34330h 96 Tests
EUR 824

Rat ASAH2 (Neutral ceramidase) ELISA Kit (CUSTOM)

ELI-48960r 96 Tests
EUR 886

Asah2 sgRNA CRISPR Lentivector (Rat) (Target 1)

K7087002 1.0 ug DNA
EUR 154

Asah2 sgRNA CRISPR Lentivector (Rat) (Target 2)

K7087003 1.0 ug DNA
EUR 154

Asah2 sgRNA CRISPR Lentivector (Rat) (Target 3)

K7087004 1.0 ug DNA
EUR 154

ASAH2 3'UTR Luciferase Stable Cell Line

TU001220 1.0 ml
EUR 1521

Asah2 3'UTR GFP Stable Cell Line

TU250951 1.0 ml Ask for price

Asah2 3'UTR Luciferase Stable Cell Line

TU200951 1.0 ml Ask for price

Asah2 3'UTR GFP Stable Cell Line

TU152183 1.0 ml Ask for price

Asah2 3'UTR Luciferase Stable Cell Line

TU102183 1.0 ml Ask for price

ASAH2 3'UTR GFP Stable Cell Line

TU051220 1.0 ml
EUR 1521

ASAH2 Protein Vector (Human) (pPB-C-His)

PV049013 500 ng
EUR 481

ASAH2 Protein Vector (Human) (pPB-N-His)

PV049014 500 ng
EUR 481

ASAH2 Protein Vector (Human) (pPM-C-HA)

PV049015 500 ng
EUR 481

ASAH2 Protein Vector (Human) (pPM-C-His)

PV049016 500 ng
EUR 329

ASAH2 Protein Vector (Human) (pPB-His-MBP)

PV324138 500 ng
EUR 481

ASAH2 Protein Vector (Human) (pPB-His-GST)

PV324139 500 ng
EUR 481

ASAH2 Protein Vector (Rat) (pPB-C-His)

PV254814 500 ng
EUR 1166

ASAH2 Protein Vector (Rat) (pPB-N-His)

PV254815 500 ng
EUR 1166

ASAH2 Protein Vector (Rat) (pPM-C-HA)

PV254816 500 ng
EUR 1166

ASAH2 Protein Vector (Rat) (pPM-C-His)

PV254817 500 ng
EUR 1166

ASAH2 Protein Vector (Mouse) (pPB-C-His)

PV156550 500 ng
EUR 1065

ASAH2 Protein Vector (Mouse) (pPB-N-His)

PV156551 500 ng
EUR 1065

ASAH2 Protein Vector (Mouse) (pPM-C-HA)

PV156552 500 ng
EUR 1065

ASAH2 Protein Vector (Mouse) (pPM-C-His)

PV156553 500 ng
EUR 1065

Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit

20-abx156834
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) CLIA Kit

20-abx494694
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit

SEE689Hu-10x96wellstestplate 10x96-wells test plate
EUR 4731.5
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) in serum, plasma, tissue homogenates and other biological fluids.

Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit

SEE689Hu-1x48wellstestplate 1x48-wells test plate
EUR 477.3
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) in serum, plasma, tissue homogenates and other biological fluids.

Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit

SEE689Hu-1x96wellstestplate 1x96-wells test plate
EUR 639
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) in serum, plasma, tissue homogenates and other biological fluids.

Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit

SEE689Hu-5x96wellstestplate 5x96-wells test plate
EUR 2575.5
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) in serum, plasma, tissue homogenates and other biological fluids.
Right here, we current suggestions from the GOT-IT working group, which have been designed to assist educational scientists and funders of translational analysis in figuring out and prioritizing goal evaluation actions and in defining a crucial path to achieve scientific targets in addition to targets associated to licensing, partnering with {industry} or initiating medical growth programmes. Primarily based on units of guiding questions for various areas of goal evaluation, the GOT-IT framework is meant to stimulate educational scientists’ consciousness of things that make translational analysis extra sturdy and environment friendly, and to facilitate academia-industry collaboration.

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